E1

First

Round

Second

Round

Fca RH Locus

Map Pos.

R.F.

GB Acc. Number

LGD clone #

Mapping Method

Hsa Chrom.

Hsa Homolog

GB4-RH

G3-RH

HsaCytogen.

Forward Primer

Reverse Primer

Forward Primer

Reverse Primer

KIAA0623

0.0

0.22

AW646906

EST-TSF7

RH

17

KIAA0623

105.07-109.27

GGTTCGAACTGAGAGCCAAG

CCTGGTGACTACCAAAGACCA

FCA140

7.3

0.20

see microsatellites

RH/GL

see microsatellites

see microsatellites

Fc.15192

35.9

0.22

AW646907

EST-TCF2

RH

17

Hs.15192

103.64

GCGAGATGGGAACAGAGAAC

GACGTTCAGGAGCAAAATGG

TP53

102.9

0.15

D26608

RH/SCH/FISH

17

TP53

53.63-54.04

17p13.1

CGCCATAAAAAGCCAATGTT

CAAGGGTGAGGGTGTCAGTT

break in map

HIC-1

0.0

0.44

AW646908

EST-TDG10

RH

17

HIC-1

17.65

CAGGGTCCTTGGTTACTGCT

GGCTGGAACGTGGTCTCTT

F124

41.6

0.58

see microsatellites

RH/GL

see microsatellites

see microsatellites

CRYBA1

51.2

0.54

AF114707

RH

17

CRYBA1

17q11.2-q12

Lyons et al., 1997

Lyons et al., 1997

CTGTATACCCTCTCTTACCG

NF1

74.4

0.55

U82189

RH

17

NF1

271.43-273.24

1714

17q11.2

Lyons et al., 1997

Lyons et al., 1997

TTGGCACGTAAGACAAGCAA

TTTGTCAGAACTTCCCATAAATGT

Fc.8179

93.0

0.65

AW646909

EST-TAG12

RH

17

Hs.8179

CCTCCTGACACAGACACGAA

TGAGGACACATTCCCAGTGA

Fc.3447

109.5

0.69

AW646910

EST-TAH11

RH

17

Hs.3447

284.02-.82

1765

CAAGCCTGTGTCAGAAACCA

ACCTCAAGGACTGGGTGGAT

break in map

FCA082

0.0

0.78

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

SUPT4H1

7.0

0.77

AW646911

EST-TGF7

RH

17

SUPT4H1

372.55-374.96

17q21-q23

TGCTGTTCATTTGTCGGAAA

TTTTCCCCCACCTTGATACC

MTMR4

9.2

0.79

AW646912

EST-TSE3

RH

17

MTMR4

372.92-375.36

CATGTCTGTGGTTGGGACAG

TTCACCTTTTATACACCAATGGA

FCA567

32.6

0.77

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

HOXB@

61.2

0.74

Human EST

RH

17

HOXB@

339.06-.17

17q21-q22

Chen et al., 1999

Chen et al., 1999

Fc.25934

77.5

0.73

AW646913

EST-TSE7

RH

17

Hs.25934

351.56

GGCTCCTTTTATTTCCACTGC

GGGCACAAGCGACTAAACA

CSF3

88.6

0.72

Y08558

RH

17

CSF3

306.88-309.38

17q11.2-q12

GCTATCAACATCTGGCAGCA

GATTTTCAGGGCTTGGTGAA

STAT5B

95.1

0.71

AW646914

EST-TSC12

RH

17

STAT5B

303.62-309.06

17q11.2

CTCTGGCGAGGACGAAGA

GACCTATGGACAGTCAGTGGA

FCA298

97.2

0.70

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

Fc.94722

106.2

0.70

AW646915

EST-TBB9

RH

17

Hs.94722

327.45

CATTGCAGAGTGCTTTTGTCC

TCTGATTACCCACACCACACA

MYL4

117.3

0.71

RH

17

MYL4

333.26-335.67

2549

17q21-qter

Lyons et al., 1997

Lyons et al., 1997

AGACAGGGCCTGAATCAGGA

HIS1

131.0

0.73

AW646916

EST-TDC2

RH

17

HIS1

329.99

GGACAGTTTTCAATTCGCTGA

AGGCACTAAGCAGGCACACT

Fc.182584

133.1

0.74

AW646917

EST-TGA9

RH

17

Hs.182584

319.32-329.15

AAGACATTCCCTCCCACCTC

GGATGGTTGGAGTCAGAGGA

Fc.19235

159.9

0.74

AW646918

EST-TCH8

RH

17

Hs.19235

405.19-405.53

GCCATCATGAATCAGCAGAA

TTAAAGGGCGAATGATTTGG

GH1

133.1-173.8

0.65

AZ081278

RH

17

GH1

405.30

17q22-q24

Lyons et al., 1997

Lyons et al., 1997

SCN4A

173.8

0.70

AZ081279

RH

17

SCN4A

405.48-.53

17q23-25.3

Jiang et al., 1998

Jiang et al., 1998

GTGGGTGTAAGTGTGCGTGT

DDX5

184.3

0.75

AW646919

RH

17

DDX5

405.3-405.81

17q21

Jiang et al., 1998

Jiang et al., 1998

GCCTCCAGAGGGCTAGGTTA

TGCCTAGGCCTTGCCAAA

FCA312

200.1

0.77

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

H3F3B

240.0

0.91

AW646920

EST-TBE5

RH

17

H3F3B

482.58-483.13

17q25

TGCATGCATCATGAGAAGCAAGAAA

CCTCAGTGATGTGAGCACTGTT

TK1

267.3

1.00

RH

17

TK1

501.01

17q23.2-25.3

GCTGGATGGGACCTTCCAGA

GGCTTCTCGGAAGCACTCCA

ACTG1

306.9

0.89

AW646921

EST-TDA5

RH

17

ACTG1

521.58-544.19

4220

17q25

CTGGGGCCTAATGTTCTCAC

TTGGAAAACGGTTTGCATTT

FCA113

328.4

0.85

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

P4HB

339.5

0.84

AZ081280

RH

17

P4HB

530.96

17q25

Lyons et al., 1997

Lyons et al., 1997

ACGCTCAGCTCAGACACCTT

COL1A1

SCH

17

COL1A1

339.6-354.45

17q221.3-q22

rRNA

SCH

rRNA