D3

First

Round

Second

Round

Fca RH Locus

Map Pos.

R.F.

GB Acc. Number

LGD clone #

Mapping Method

Hsa Chrom.

Hsa Homolog

GB4-RH

G3-RH

HsaCytogen.

Forward Primer

Reverse Primer

Forward Primer

Reverse Primer

Fc.23794

0.0

0.39

AW646891

EST-TDA12

RH

Hs.23794

TTCCAGATGACATGGCAAAC

CCCCATGGTCTTTCTCTGTT

FCA137

47.7

0.39

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

MAPKAPK5

72.9

0.41

AW646892

EST-TAF3

RH

12

MAPKAPK5

427.21

GGAACTGAGCTTTTCTTACAAACA

AAATCCATAATTTCATTTCCACA

ALDH2

102.8

0.51

AF114704

RH

12

ALDH2

427.1

4788

12q24.2

Murphy et al., 1999a

Murphy et al., 1999a

Fc.7729

124.1

0.53

AW646893

EST-TED2

RH

Hs.7729

443.23

TCTTGCCACCAAGTGTGACT

GTCACCCAGATCCTGTTTCC

TCF1

139.1

0.51

AF114705

RH

12

TCF1

466.94

12q24.3

Murphy et al., 1999a

Murphy et al., 1999a

PBP

157.9

0.53

AW646894

EST-TAB9

RH

12

PBP

462.88

CCCTTCAAGAGTCTTTGATGCT

GTACCAGGATGGGGTCATCA

IGL@

172.9

0.53

U85410

RH/SCH/FISH

22

IGL@

45.82

22q11.2

Murphy et al., 1999a

Murphy et al., 1999a

FCA523

196.2

0.52

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

GNAZ

201.4

0.50

RH

22

GNAZ

43.77

22q11.2

Murphy et al., 1999a

Murphy et al., 1999a

SLC5A1

216.0

0.37

AF114706

RH

22

SLC5A1

92.83

22q13.1

Jiang et al., 1998

Jiang et al., 1998

SLC25A1

284.0

0.70

AW646895

EST-TDA3

RH

22

SLC25A1

2.63

22q11

CGGAGGCAGCTTTGGTAG

CGGAGGCAGCTTTGGTAG

FCA026

304.7

0.75

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

LAMA1

342.3

0.62

AW646896

EST-TCD3

RH

18

LAMA1

25.76

18p11.32-11.22

CAGAAAAGCAAACACCGCTTG

AGGCAGTTTTGCTTTACGTCA

FCA132

375.6

0.54

see microsatellites

RH/GL

see microsatellites

see microsatellites

TYMS

400.9

0.44

AZ081274

RH

18

TYMS

13.7-15.13

18p11.31-11.22

Jiang et al., 1998

Jiang et al., 1998

GGGTGTACCCTTCAACATCG

AGCTGCAAGGTGAGCAAGTT

CDH2

435.5

0.50

Human EST

RH

18

CDH2

178.24

18q12.1

Chen et al., 1999

Chen et al., 1999

TTR

468.9

0.36

AZ081275

RH

18

TTR

248.08

18q12.1

Lyons et al., 1997

Lyons et al., 1997

ACCTCTGCCACTCAGCTTGT

AACCCAGTGCACCAAAGCTA

FCA441

494.9

0.42

see microsatellites

RH/GL

see microsatellites

see microsatellites

FCA127

509.7

0.43

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

FCA603

530.9

0.45

see microsatellites

RH/GL

see microsatellites

see microsatellites

HNRPDL

571.7

0.50

AW646897

EST-TCA3

RH

4

HNRPDL

444.39-449.13

CCACCATGGAGGACATGAAC

TCAATTTGCCATCCAGTTTG

FCA623

610.2

0.38

see microsatellites

RH/GL

see microsatellites

see microsatellites

Fc.182923

638.2

0.45

AW646898

EST-TAC9

RH

15

Hs.182923

3339

GTCATGCCACGGAATTATCC

CGTCACTCTGACACCTCACG

Fc.7922

658.9

0.44

AW646899

EST-TGB3

RH

18

Hs.7922

461.97-462.18

AGCAGAAACACTGTCCACGA

AGGAAAGAACACATGGCTCA

RD114-8H

673.9

0.47

unpublished

RH/SCH

TGCTGGGACAGCTGGATAG

TTAAGGTTTCGATCCATTTTGA

FCA277

687.1

0.41

see microsatellites

RH/GL

see microsatellites

see microsatellites

FCA221

712.7

0.30

see microsatellites

RH/GL

see microsatellites

see microsatellites

FCA006

731.6

0.18

see microsatellites

RH/GL

see microsatellites

see microsatellites

YES1

SCH

18

YES1

0.58-13.7

18p11.31-11.21