D1

First

Round

Second

Round

Fca RH Locus

Map Pos.

R.F.

GB Acc. Number

LGD clone #

Mapping Method

Hsa Chrom.

Hsa Homolog

GB4-RH

G3-RH

HsaCytogen.

Forward Primer

Reverse Primer

Forward Primer

Reverse Primer

Fc.21970

0.0

0.36

AW646876

EST-TSF1

RH

11

Hs.21970

230.61

2707

GGGACTCTGCCTCGTTCTCT

CTCTGCCTCTGCCTCTGC

SAP145

34.0

0.32

AW646877

EST-TBG11

RH

11

SAP145

247.63

CCTGAAGAGTTGGAGCTGGA

GCATCCAGACAAAGAGCTGA

ROM1

41.5

0.29

RH

11

ROM1

228.95

11q13

Lyons et al., 1997

Lyons et al., 1997

CTCCTCTATAGCGGGCACCT

ACCAGTCCTGTGCCCAAA

Fc.132216

53.6

0.32

AW646878

EST-TBD10

RH

Hs.132216

CTCCAGAACCTCTCCGACTG

ACTACCACATGGGGCCACT

HRAS

87.2

0.38

U62092

RH/SCH

11

HRAS

22.6

11p15.5

TCATCCAGAACCACTTCGTG

ACTTGGTGTTGTTGATGGCA

Fc.19121

103.3

0.37

AW646745

EST-HAB6

RH

11

Hs.19121

2.4-7.24

CGACTGGGCTGTTTATTTGC

AGTTTTAATCGCCGAGGATG

KCNQ1

122.5

0.32

AF013961

RH

11

KCNQ1

4.74-19.14

11p15.5

GGAGCTCATCACCACCTTGT

CGTAGCTGCCAAACTCCACT

FCA239

173.2

0.17

see microsatellites

RH/GL

see microsatellites

see microsatellites

FCA180

194.5

0.24

see microsatellites

RH/GL

see microsatellites

see microsatellites

CAT

205.3

0.28

U82180

RH

11

CAT

128.0-131.6

1650

11p13

Lyons et al., 1997

Lyons et al., 1997

GAAAATCCAAAGAGGCACCA

FSHB

240.8

0.33

AZ081269

RH

11

FSHB

113.44-117.83

1377

11p13

Lyons et al., 1997

Lyons et al., 1997

GCAATGGACATTTCAGGTGAC

AATTCCTGGACCTCCTCTGC

WT1

247.5

0.34

AZ081270

RH

11

WT1

114.28-120.18

11p13

Jiang et al., 1998

Jiang et al., 1998

CACCAAAGGAGACACACAGG

GGGGAGGGAATTATTGGAAA

SAA

258.9

0.35

AF136718

RH

11

SAA1

65.71-71.39

800

11p15.1

GCCAATTACATAGGTGCAGACA

TTCCGTGCCTGAAAAAGTCT

LDHA

270.6

0.30

AZ081271

RH/SCH

11

LDHA

65.44

800

11p15.4

Lyons et al., 1997

Lyons et al., 1997

TTTGGTCCAGCGTAATGTGA

TGCCATCAGTGTTACTCAAGC

FCA698

288.3

0.27

see microsatellites

RH/GL

see microsatellites

see microsatellites

HBB

324.8

0.32

AF114701

RH/SCH

11

HBB

36.42-36.52

11p15.5

Lyons et al., 1997

Lyons et al., 1997

FCA347

336.5

0.38

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

FCA067

336.5-363.8

0.26

see microsatellites

RH/GL

see microsatellites

see microsatellites

FCA076

363.8

0.46

see microsatellites

RH/GL

see microsatellites

see microsatellites

TYR

400.2

0.50

U40716

RH/SCH

11

TYR

301.53

11q14-q21

GCTTTATGTGATGGAACTCCA

CAGCTTTATCCATGGAATCC

ACAT1

457.4

0.43

AW646746

EST-HAC8

RH

11

ACAT1

365.75-366.62

4763

11q22.3-23.1

TGGAGAGCATGTCCAACGCTC

ATGGATTTTATTGTAAGACGTCAGTCAG

Fc.39143

497.3

0.46

AW646880

EST-TBF11

RH

11

Hs.39143

373.38-373.8

TTATTCCGCAGAGCCCTAAA

TGTGTGGGCTTAAGAGATTGG

Fc.4278

514.7

0.49

AW646879

EST-TGD2

RH

11

Hs.4278

373.36

CGCACACTGATGAACCAAAG

CAGCTCGGGAATGAAATGAC

FCA193

537.8

0.46

see microsatellites

RH/GL/SCH

see microsatellites

see microsatellites

Fc.195800

550.9

0.50

FcaEST-TBF1

EST-TBF1

RH

11

Hs.195800

376.89-380.55

11q22.3-23

ATTTCAGGAGCTCGGTGTTG

GGAACGGAGAACTTAAAGATGG

THY1

580.2

0.44

U82192

RH

11

THY1

387.65-388.06

5331

11q22.3-23

Lyons et al., 1997

Lyons et al., 1997

GGTGGTAGACTGGCCAGAGA

Fc.23690

607.1

0.52

AW646881

EST-TGD10

RH

Hs.23690

AAGTGCACACCAGCCCTTT

CTTGGAGCCTGAAGCTTTCT

SP17

635.3

0.55

AW646882

EST-TGC11

RH

SP17

GCATGTGTTCAATGGCATGT

GCTCCCCAGAAAAATGTGAA

Fc.12293

647.1

0.57

AW646883

EST-TGG5

RH

11

Hs.12293

417.73-419.82

GCCATTGAGCTTAATTGAAAAA

TGTGTCCACACAGCAGATCA

Fc.148565

663.6

0.48

AW646884

EST-TBF8

RH

Hs.148565

ACTTGCTTGGGACCATTCTG

ATCATGACCTGAGCCGAAGT

FCA087

693.5

0.43

see microsatellites

RH/GL

see microsatellites

see microsatellites

ACP2

SCH

11

ACP2

167.85

11p12-p11

FGF3

SCH

11

FGF3

3006

11q13

HMBS

SCH

11

HMBS

382.36

5331

11q23.3

ETS1

SCH

11

ETS1

419.62

11q23.3

GANAB

SCH

11

GANAB

11q13-qter