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| A. Computer-generated
Image of a Model Chromatin Fiber. | B. Simulated
SFM Image of the Model Chromatin Fiber in 'A'. | Experimental
SFM Image of a Glutaraldehyde-fixed Chromatin Fiber |
Using the following constraints:
(1) the linker histones H1 and H5 help maintain the DNA entry/exit angle
on the nucleosome; (2) the DNA linkers between nucleosomes are rigid and
straight under the low ionic conditions used here; and (3) the DNA linker
length varies within biochemically defined limits (constant linkers would
generate a regular helical fiber of nucleosomes); we have been able to
model at a first approximation the irregular, three-dimensional character
of chromatin fibers.
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