NCI Core Fluorescence
Imaging Facility

The NCI Fluorescence Imaging Facility houses equipment for 2D or 3D imaging of specimens, as well as for 2D or 3D time-lapse imaging of living specimens. High resolution images can be obtained by confocal microscopy or deconvolution. The microscopes are highly automated, and data are recorded digitally. Special expertise is available for assistance with deconvolution microscopy, as well as live cell imaging. Facility personnel are also experienced in protein colocalization, in FISH analysis, in fluorescence photobleaching techniques, such as FRAP and FLIP, and also provide expertise and software for quantitative analysis of images. A new interest of the Facility is in the development of techniques for fluorescence resonance energy transfer (FRET) microscopy.