| The mammalian cell nucleus is the site of gene expression. The interior of the nucleus is highly organized and contains distinct protein subcompartments and chromatin domains. It is likely that this internal nuclear architecture has critical regulatory functions in gene expression. How nuclear structure affects gene expression is largely unclear. We use molecular methods in conjunction with microscopy techniques and kinetic modeling to study the biogenesis of these compartments and their role in gene expression in living cells. In addition, we are using in vivo microscopy and computer modeling to uncover the dynamic properties of proteins and macromolecular complexes in vivo. |
