NCI Laboratory of Receptor Biology and Gene Expression NIH

Fluorescence Imaging Group

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    Research Overview
    Education
    Publications
    Affiliations
    Curriculum Vitae (.pdf)
Tim Stasevich, Post-doctoral Research Fellow

Contact Information

Address: Timothy J. Stasevich, Ph.D.
LRBGE-National Cancer Institute
The National Institutes of Health
41 Library Drive
Bethesda, MD 20892 		    Email: stasevicht@mail.nih.gov
    Phone: (301) 496 - 4952
    Fax: (301) 496 - 4951
    Office: B513 Bldg. 41

Research Overview:

I work with Jim McNally in the Laboratory of Receptor Biology and Gene Expression at NIH. I am currently studying how molecules bind DNA and/or chromatin in vivo using FRAP (fluorescence recovery after photobleaching) and FCS (fluorescence correlation spectroscopy).

Many biological molecules are composed of multiple binding domains that can simultaneously bind a substrate in sequential steps to increase the overall bond strength. I have recently developed a systematic procedure for analyzing these "multivalent" molecules in vivo and am using it to dissect the trivalent binding of the linker histone H1 to chromatin. This procedure relies on a novel extension of FRAP to produce spatio-temporal images of recoveries for improved fitting and binding parameter estimates.

Before working at NIH, I received my Ph.D. in physics at the University of Maryland, where I worked with Prof. Ted Einstein studying the dynamics of nanoscale structures on crystalline surfaces using the tools of stastistical mechanics.

Education:
Publications:

[16] A General Procedure for Dissecting the Binding Mechanisms of Complex Molecules with Application to H1-Chromatin Binding, T. J. Stasevich, F. Mueller, D. T. Brown, and J. G. McNally, preprint (2009).
[15] Direct Measurement of Association and Dissociation Rates of DNA Binding in Live Cells by Fluorescence Correlation Spectroscopy, A. Michelman-Ribeiro, D. Mazza, T. Rosales, T. J. Stasevich, H. Boukari, V. Rishi, C. Vinson, J. R. Knutson, and J. G. McNally, accepted for publication in Biophyical J. (2009).
[14] Impurity Decoration for Crystal Shape Control: C60 on Ag(111), T. J. Stasevich, C. Tao, W. G. Cullen, T. L. Einstein, and E. D. Williams, Phys. Rev. Lett. 102, 085501 (2009).
[13] Temperature Dependence of Si(111) Absolute Line Tension, M. Man, T. J. Stasevich, F. Szalma, T. L. Einstein, and M. Altman, Phys. Rev. B 77, 115424 (2008).
[12] Concurrent Fast and Slow Cycling of a Transcriptional Activator at an Endogenous Promoter, T. S. Karpova, M. J. Kim, C. Spriet, K. Nalley, T. J. Stasevich, Z. Kherrouche, L. Heliot, and J. G. McNally, Science 25, 466 (2008).
[11] Sensitivity of Short-Range Trio Interactions to Lateral Relaxation of Adatoms: Challenges for Detailed Lattice-Gas Modeling, Rajesh Sathiyanarayanan, T. J. Stasevich, and, T. L. Einstein, Surf. Sci. (2008), doi: 10.1016/j.susc.2008.01.022
[10] Facet-edge Fluctuations with Periphery Diffusion Kinetics, M. Degawa, T. J. Stasevich, A. Pimpinelli, T. L. Einstein, E. D. Williams, Surf. Sci. 601, 3979 (2007).
[9] Metal/Molecule Interface Fluctuations, C. Tao, T. J. Stasevich, W. G. Cullen, T. L. Einstein, and E. D. Williams, Nano Lett. 7, 1495 (2007). [Highlighted in Nature 446, 472 (2007)]
[8] Analytic Formulas for the Full Orientation Dependence of Step Stiffness and Line Tension, T. J. Stasevich and T. L. Einstein, Multiscale Modeling and Simulation 6, 90 (2007).
[7] Modeling the Anisotropy of Step Fluctuations on Surfaces: Theoretical Step Stiffness Confronts Experiment, T. J. Stasevich, Ph.D. Thesis, Dept. of Physics, University of Maryland, College Park.
[6] Distinctive Fluctuations in a Confined Geometry, M. Degawa, T. J. Stasevich, W. G. Cullen, Alberto Pimpinelli, T. L. Einstein, and E. D. Williams, Phys. Rev. Lett. 97, 080601 (2006).
[5] Step Fluctuations on Ag(111) Surfaces with C60, C. Tao, T. J. Stasevich, T. L. Einstein, and E. D. Williams, Phys. Rev. B 73, 125436 (2006).
[4] Extended Lattice Gas Interactions of Cu on Cu(001) and Cu(111): Ab-initio Evaluation and Implications, T. J. Stasevich, T. L. Einstein, and S. Stolbov, Phys. Rev. B 73, 115426 (2006).
[3] Low-Temperature Orientation Dependence of Step Stiffness on {111} Surfaces, T. J. Stasevich, H. Gebremariam, and T. L. Einstein, Phys. Rev. B 71, 245414 (2005).
[2] Effects of Next-Nearest-Neighbors on the Orientation Dependence of Step Stiffness: Reconciling Theory with Experiment for Cu(001), T. J. Stasevich, T. L. Einstein, R. K. P. Zia, M. Giesen, H. Ibach, and F. Szalma, Phys. Rev. B 70, 245404 (2004).
[1] Crossover from the Exact Factor to the Boltzmann Factor, J. J. Prentis, A. E. Andrus and T. J. Stasevich, Am. J. Phys. 67, 508 (1999).

Affiliations