Fluorescence Imaging Group
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Contact Information Research Overview Education Publications Affiliations Curriculum Vitae (.pdf) |
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Min Jeong Kim, Research Assistant |
Contact Information
| Address: |
Min Jeong Kim LRBGE-National Cancer Institute The National Institutes of Health 41 Library Drive Bethesda, MD 20892 |
Email: kimm2@mail.nih.gov Phone: (301) 451 - 4731 Fax: (301) 496 - 4951 Office: B513 Bldg. 41 |
Research Overview:
I work with Tatiana Karpova in the Laboratory of Receptor Biology and Gene Expression at NIH. We are studying dynamic interactions between a transcription factor, Ace1p, and an endogenous promoter, CUP1, in Saccharomyces cerevisiae, using yeast genetic engineering, biochemistry, live cell imaging, and FRAP (Fluorescence Recovery After Photobleaching).
In our recent Science paper we demonstrated that fast cycling and slow cycling occur simultaneously on CUP1, that fast cycling is the very rapid exchange of Ace1p molecules on the CUP1 promoter and is associated with the initiation of transcription, and that slow cycling reflects a modulation in the fraction of accessible promoters rather than recruitment and release of the transcriptional activator.
We are currently working to understand how slow cycling plays a role in the regulation of transcription.
Education:
Publications:
| [1] | Concurrent Fast and Slow Cycling of a Transcriptional Activator at an Endogenous Promoter, T. S. Karpova, M. J. Kim, C. Spriet, K. Nalley, T. J. Stasevich, Z. Kherrouche, L. Heliot, and J. G. McNally, Science 25, 466 (2008). |
Affiliations