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Contact information Recent publications Curriculum vitae Laboratory of Molecular Biology |
Biography: Dr. Garges received her Ph.D. in Microbiology in 1983 from the University of Maryland. She did her postdoctoral research at NIH and has remained at NCI. Dr. Garges serves on the editorial board of the Journal of Bacteriology.Research: We have been focusing on two aspects in the pathway to CRP activation of transcription: (1) CRP conformation--CRP must become activated as a DNA binding protein. This is effected by cAMP binding to the protein and causing an allosteric change. We have studied the cAMP-induced allosteric change by mutational analysis, and, among the different classes of mutants that we have isolated, we found some CRP mutants that are defective in the allosteric change. We have recently isolated intragenic suppressor mutations that restore the wild type phenotype to the allosteric-defective mutants. From the location of these suppressor mutations, and the characterization of the mutant proteins, we are learning how amino acids are communicating with each other within the protein to effect the cAMP-induced conformational change. (2) Transcription activation by CRP--we have addressed the mechanism for transcription activation by CRP by studying the kinetics of transcription initiation at a promoter that is positively regulated by CRP. We have developed a new program to analyze the kinetics, and we have shown that CRP is working in at least two steps in the initiation process.Recent Publications: |
Contact Information: Laboratory of Molecular Biology,NCI, NIHBuilding 37, Room 2E0637 CONVENT DR MSC 4255BETHESDA MD 20892-4255Phone: 301-496-1019Fax:301-496-2212Email: sgarges@helix.nih.gov
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