Summary of results for microarray
analysis
of stress responses(1)
| Array(2) | Targets | Treatment | Induced(3) | Reduced(4) | 99% Conf.(5) |
| 1.2K Chip | 1238 | 20 Gy + 4hr | 38 | 11 | .63-2.37 |
| 1238 | 6 hr hypoxia | 135 | 19 | .51-2.47 | |
| 1238 | 24 hr hypoxia | 202 | 8 | .55-2.38 | |
| 5K Chip | 5408 | 2 Gy + 3hr | 67 | 21 | .68-2.31 |
| 5408 | 2 Gy + 24 hr | 161 | 26 | .69-1.89 |
notes:
1Results are summarized for microarray hybridizations conducted in ML-1 cells. Many of the results for row 1 and 3 have been verified by quantitative single-probe hybridization.2 Two different micorarrays (chips) used contained only limited overlap in the genes represented.
3 The number induced refers to cDNA clones showing significant induction (>99% confidence).
4 For cDNA clones showing a significant reduction, values are shown for those having at least a 2-fold reduction in expression compared to untreated cells; all these values exceeded 99% confidence.
5 Values represent range of relative expression of irradiated sample compared to untreated control; e.g. only targets showing an increase in the relative mRNA level of 2.37-fold or more were scored as induced in the first row.
see: Amundson, S.A., Bittner, M., Yidong, C., Trent, J., Meltzer, P., and Fornace, A.J., Jr. Fluorescent cDNA microarray hybridization reveals complexity and heterogeneity of cellular genotoxic stress responses. Oncogene 18: 3666-3672, 1999.